Dynamic Conformational Behavior and Molecular Interaction Discrimination of DNA/Binder Complexes by Single-Chain Stretching in a MicroDevice
Wei-Hua Huang Dr.
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorAnatoly A. Zinchenko Dr.
Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601, Japan
Search for more papers by this authorCyril Pawlak
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorYong Chen Prof.
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorDamien Baigl Dr.
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorWei-Hua Huang Dr.
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorAnatoly A. Zinchenko Dr.
Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601, Japan
Search for more papers by this authorCyril Pawlak
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorYong Chen Prof.
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorDamien Baigl Dr.
Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorGraphical Abstract
All stretched out. We report the use of a microfluidic device to study the unfolding process of compact single-chain DNA, as well as to investigate the molecular interaction between DNA and compaction agents. It has been found that DNA molecules that were compacted by spermine could be dynamically unfolded, but DNA molecules that were compacted by polylysine could not be unfolded. The method and results might find application in DNA separation, or in the control of DNA bioactivity.
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