Volume 9, Issue 5 p. 694-697
Communication

Mutant DNA Polymerase for Improved Detection of Single-Nucleotide Variations in Microarrayed Primer Extension

Ramon Kranaster

Ramon Kranaster

Fachbereich Chemie, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140

These authors contributed equally to this work.

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Patrick Ketzer

Patrick Ketzer

Fachbereich Chemie, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140

These authors contributed equally to this work.

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Andreas Marx Prof. Dr.

Andreas Marx Prof. Dr.

Fachbereich Chemie, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany, Fax: (+49) 7531-88-5140

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First published: 14 March 2008
Citations: 13

Graphical Abstract

Mutant detects mutation. A mutant DNA polymerase, which was derived from Pyrococcus furiosus DNA polymerase (M2 in figure), was found to have improved single-nucleotide discrimination properties, and was used for selective microarrayed-primer extensions. This approach allowed multiplexed SNP detection.