Volume 9, Issue 8 p. 1201-1206
Communication

Photocontrol of Single-Chain DNA Conformation in Cell-Mimicking Microcompartments

Matthieu Sollogoub Prof.

Matthieu Sollogoub Prof.

Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France

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Samuel Guieu

Samuel Guieu

Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France

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Marie Geoffroy

Marie Geoffroy

Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402

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Ayako Yamada Dr.

Ayako Yamada Dr.

Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan

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André Estévez-Torres Dr.

André Estévez-Torres Dr.

Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan

Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan

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Kenichi Yoshikawa Prof.

Kenichi Yoshikawa Prof.

Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan

Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan

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Damien Baigl Dr.

Damien Baigl Dr.

Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402

Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan

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First published: 15 May 2008
Citations: 51

Graphical Abstract

Shedding light on DNA: Combined with the addition of a photosensitive cationic surfactant, we used light to control the single-chain higher-order structure of genomic duplex T4 DNA (166 kbp) in bulk solution and in cell-mimicking microcompartments. We demonstrate that this strategy can be used to encapsulate folded/unfolded genomic DNA inside phospholipid-coated cell-sized microdroplets with high efficiency and low DNA damage.