Photocontrol of Single-Chain DNA Conformation in Cell-Mimicking Microcompartments
Matthieu Sollogoub Prof.
Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France
Search for more papers by this authorSamuel Guieu
Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France
Search for more papers by this authorMarie Geoffroy
Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorAyako Yamada Dr.
Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Search for more papers by this authorAndré Estévez-Torres Dr.
Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
Search for more papers by this authorKenichi Yoshikawa Prof.
Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
Search for more papers by this authorDamien Baigl Dr.
Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
Search for more papers by this authorMatthieu Sollogoub Prof.
Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France
Search for more papers by this authorSamuel Guieu
Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France
Search for more papers by this authorMarie Geoffroy
Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Search for more papers by this authorAyako Yamada Dr.
Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Search for more papers by this authorAndré Estévez-Torres Dr.
Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
Search for more papers by this authorKenichi Yoshikawa Prof.
Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
Search for more papers by this authorDamien Baigl Dr.
Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
Search for more papers by this authorGraphical Abstract
Shedding light on DNA: Combined with the addition of a photosensitive cationic surfactant, we used light to control the single-chain higher-order structure of genomic duplex T4 DNA (166 kbp) in bulk solution and in cell-mimicking microcompartments. We demonstrate that this strategy can be used to encapsulate folded/unfolded genomic DNA inside phospholipid-coated cell-sized microdroplets with high efficiency and low DNA damage.
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