Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease
Dr. Ki Soo Park
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)
Search for more papers by this authorProf. Richelle C. Charles
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA)
Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA)
Search for more papers by this authorProf. Edward T. Ryan
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA)
Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA)
Search for more papers by this authorCorresponding Author
Prof. Ralph Weissleder
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)
Department of Systems Biology, Harvard Medical School, Boston, MA 02115 (USA)
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)Search for more papers by this authorCorresponding Author
Prof. Hakho Lee
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)Search for more papers by this authorDr. Ki Soo Park
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)
Search for more papers by this authorProf. Richelle C. Charles
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA)
Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA)
Search for more papers by this authorProf. Edward T. Ryan
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114 (USA)
Department of Medicine, Harvard Medical School, Boston, MA 02114 (USA)
Search for more papers by this authorCorresponding Author
Prof. Ralph Weissleder
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)
Department of Systems Biology, Harvard Medical School, Boston, MA 02115 (USA)
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)Search for more papers by this authorCorresponding Author
Prof. Hakho Lee
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 (USA)Search for more papers by this authorGraphical Abstract
Nucleic acid testing: Detection probes that regulate DNA polymerase activity were rationally designed for a rapid and cost-effective diagnosis of infectious disease. This assay system was successfully utilized to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in relatively short periods of time (see scheme).
Abstract
A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
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