Volume 2011, Issue 10 p. 1635-1639
Full Paper

Electrochemical Biosensor Assay of the Interaction between [PtCln(NH3)4–n](2–n) (n = 0–4) Complexes and ds-DNA

Mauro Ravera

Mauro Ravera

Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale “Amedeo Avogadro”, Viale Teresa Michel 11, 15121 Alessandria, Italy, Fax: +39-0131-360250

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Elisabetta Gabano

Elisabetta Gabano

Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale “Amedeo Avogadro”, Viale Teresa Michel 11, 15121 Alessandria, Italy, Fax: +39-0131-360250

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Manuele Sardi

Manuele Sardi

Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale “Amedeo Avogadro”, Viale Teresa Michel 11, 15121 Alessandria, Italy, Fax: +39-0131-360250

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Manuela Alessio

Manuela Alessio

Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale “Amedeo Avogadro”, Viale Teresa Michel 11, 15121 Alessandria, Italy, Fax: +39-0131-360250

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Domenico Osella

Domenico Osella

Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale “Amedeo Avogadro”, Viale Teresa Michel 11, 15121 Alessandria, Italy, Fax: +39-0131-360250

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First published: 23 February 2011
Citations: 4

Presented at the 10th Workshop on Pharmaco-Bio-Metallics, 28–29 October, 2010, Pozzuoli (Naples), Italy

Graphical Abstract

The interaction between double-stranded DNA (ds-DNA) and six neutral, anionic or cationic Pt complexes [PtCln(NH3)4–n](2–n) (n = 0–4) was evaluated by using an electrochemical DNA biosensor. This interaction was measured as a function of the variation in the guanine oxidation signal of themetal–DNA adduct deposited onto theelectrode.

Abstract

We report on the results of electrochemical DNA biosensor assay of the interaction between double-stranded DNA (ds-DNA) and a series of six neutral, anionic or cationic Pt complexes of the general formula [PtCln(NH3)4–n](2–n) [n = 4, 1; n = 3, 2; n = 2, isomers cisplatin (3) and transplatin (4); n = 1, 5; n = 0, 6]. The ability of the electrophilic PtII agents generated in solution to interact with DNA, and hence to form Pt–DNA adducts, was measured as a function of the decrease in the guanine oxidation signal recorded on a screen-printed electrode by using square wave voltammetry. Hydrolysis of the platinum complexes was studied by using time-resolved RP-HPLC and conductivity measurements to determine the aquation rate, which modulates the formation of the electrophilic agent prone to quickly interact with DNA. Our findings indicate that, if time is allowed for sufficient hydrolysis to occur, the interaction of these PtII complexes with ds-DNA follows the order 2 > 1 > 34 > 5 >> 6.