Volume 7, Issue 22 e202200506
Research Article

Synthesis, Optical Properties, Preliminary Antimycobacterial Evaluation and Docking Studies of Trifluoroacetylated 3-Pyrrolyl Boron-Dipyrromethene

Matvey Horetski

Matvey Horetski

Department of Macromolecular Compounds, Belarusian State University, 14 Leningradskaya Street., Minsk, 220030 Belarus

Search for more papers by this author
Anna Gorlova

Anna Gorlova

Department of Natural Sciences, Novosibirsk State University, 1 Pirogova Street., Novosibirsk, 630090 Russia

Search for more papers by this author
Dr. Renata Płocińska

Dr. Renata Płocińska

The Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa Street., Lodz, 93-232 Poland

Search for more papers by this author
Dr. Anna Brzostek

Dr. Anna Brzostek

The Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa Street., Lodz, 93-232 Poland

Search for more papers by this author
Dr. Yaroslav Faletrov

Dr. Yaroslav Faletrov

Department of Macromolecular Compounds, Belarusian State University, 14 Leningradskaya Street., Minsk, 220030 Belarus

Search for more papers by this author
Dr. Jarosław Dziadek

Dr. Jarosław Dziadek

The Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa Street., Lodz, 93-232 Poland

Search for more papers by this author
Prof. Vladimir Shkumatov

Corresponding Author

Prof. Vladimir Shkumatov

Department of Macromolecular Compounds, Belarusian State University, 14 Leningradskaya Street., Minsk, 220030 Belarus

Search for more papers by this author
First published: 09 June 2022
Citations: 2

Graphical Abstract

Bright, facile and bioactive! Insertion of trifluoroacetyl group into the structure of 3-pyrrolyl boron dipyrromethene (BODIPY) undergoes with a high yield and leads to a new product with considerably increased absorption and emission intensities. DFT calculations results helped to point out the role of intramolecular rotations in compounds’ bright fluorescence. Obtained BODIPY dye performs distinct antimycobacterial activity and its possible target proteins are proposed with molecular modeling.

Abstract

The article provides the route to trifluoroacetylated fluorescent boron dipyrromethene dye (2). The compound performs intensive absorption and emission bands in near-red wavelength region. Photophysical properties of 2 are investigated in different solvents. A notable increase of fluorescence quantum yield after trifluoroacetyl group insertion connected with a change in intramolecular interactions parameters. The role of molecular geometry aspects in observed photophysical properties and molecular fragments rotation energy barriers are explained by quantum-mechanical calculations. 2 performs considerable antimycobacterial activity. According to molecular docking simulations, 2 is capable of affinity binding in the active sites of mycobacterial MycP1 and MycP3 serine proteases. The geometries of final ligand-protein complexes show the possibility of protein active site covalent modification at the serine residue of the catalytic triad. At lower concentrations, 2 can act as a fluorescent dye for microscopy analysis and corresponding staining patterns are shown for Mycobacterium smegmatis and Staphylococcus aureus cells.

Conflict of interest

The authors declare no conflict of interest.

Data Availability Statement

The data that support the findings of this study are available in the supplementary material of this article.