Volume 12, Issue 3 p. 661-671
Full Paper

Urban Particulate Matter-Induced Decomposition of S-Nitrosoglutathione Relevant to Aberrant Nitric Oxide Biological Signaling

Dr. Maria Oszajca

Corresponding Author

Dr. Maria Oszajca

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

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Anna Wądołek

Anna Wądołek

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

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James Hooper

James Hooper

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

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Dr. Małgorzata Brindell

Dr. Małgorzata Brindell

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

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Prof. Rudi van Eldik

Prof. Rudi van Eldik

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

Department of Chemistry and Pharmacy, University of Erlangen-Nuremberg, Egerlandstr. 1, 91058 Erlangen, Germany

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Prof. Grażyna Stochel

Corresponding Author

Prof. Grażyna Stochel

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

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First published: 14 November 2018
Citations: 7

Graphical Abstract

Signaled out: The role of the urban particulate matter (PM) in the disturbance of the S-nitrosation signaling pathway is studied by examining the interaction of PM from various sources with S-nitrosoglutathione (GSNO). Key factors in the decomposition of GSNO are identified and the influence of biological reductants is taken into account. The influence of soluble and insoluble fractions of the PM is analyzed.

Abstract

Exposure to airborne particulate matter (PM) is associated with hazardous effects on human health. Soluble constituents of PM may be released in biological fluids and disturb the precisely tuned nitric oxide signaling processes. The influence of aqueous extracts from two types of airborne urban PM (SRM 1648a, a commercially available sample, and KR PM2.5, a sample collected “in-house” in Krakow, Poland) on the stability of S-nitrosoglutathione (GSNO) was investigated. The particle interfaces had no direct effect on the studied reaction, but extracts obtained from both samples facilitated NO release from GSNO. The effectiveness of NO release was significantly affected by glutathione (GSH) and ascorbic acid (AscA). Examination of the combined influence of Cu2+, Fe3+, and reductants on GSNO stability revealed copper to be the main GSNO decomposing species. Computational models of nitrosothiols interacting with metal oxide substrates and solvated metal ions support these claims. The study stresses the importance of the interplay between metal ions and biological reductants in S-nitrosothiols decomposition.

Conflict of interest

The authors declare no conflict of interest.